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KMID : 0380020130280010048
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 1 p.48 ~ p.53
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Pretreatment of Wastepaper using Aqueous Glycerol to enhance Enzymatic Hydrolysis
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Seo Dong-Il
Kim Chang-Joon
Kim Sung-Bae
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Abstract
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Pretreatment of wastepaper using aqueous glycerol was investigated to enhance the enzymatic hydrolysis. The effects of four factors (solid/liquid ratio, glycerol concentration, acid concentration, and reaction time) on the dissolution yield, the removal of cellulose, hemicellulose and lignin, and the enzymatic digestibility were examined at 150oC. The 1/8 of solid/liquid was determined to perform the reaction uniformly, and the 93% of glycerol concentration was found to be a minimum concentration to conduct the reaction under atmospheric pressure. Also, it was found that the acid concentration and reaction time were strongly related to the dissolution yield and the removal of cellulose, hemicellulose and lignin, but moderately to the enzymatic digestibility. At an optimum condition of 150oC, 1 h and 1% acid concentration, 56% and 49% of hemicellulose and lignin, respectively, were removed, while only 4% of cellulose was removed. The enzymatic digestibility at this condition was 86%, meaning that 83% of the glucan present in the initial substrate was converted to glucose. Compared to glycerol with ethylene glycol as a pretreatment solvent, glycerol is much cheaper than ethylene glycol, but ethylene glycol is superior to glycerol in delignification.
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KEYWORD
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Enzymatic hydrolysis, Glycerol, Organosolv, Pretreatment, Wastepaper
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